U7 small nuclear RNA in C snurposomes of the Xenopus germinal vesicle.
نویسندگان
چکیده
منابع مشابه
Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes
We have examined the distribution of snRNPs in the germinal vesicle (GV) of frogs and salamanders by immunofluorescent staining and in situ nucleic acid hybridization. The major snRNAs involved in pre-mRNA splicing (U1, U2, U4, U5, and U6) occur together in nearly all loops of the lampbrush chromosomes, and in hundreds to thousands of small granules (1-4 microns diameter) suspended in the nucle...
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Small nuclear RNA genes transcribed by RNA polymerase I1 have 3 conserved sequence elements necessary for efficient transcription. The distal sequence element, DSE (-250 to -200) is an enhancer containing at least one octamer sequence motif. The DSE has been shown to function independent of orientation and to some extent position [ I ] . The proximal sequence element, PSE (-60 to -50) is essent...
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Base pairing between the 5' end of U7 small nuclear RNA (snRNA) and the histone downstream element (HDE) in replication-dependent histone pre-mRNAs is the key event in 3'-end processing that leads to generation of mature histone mRNAs. We have cloned the Drosophila U7 snRNA and demonstrated that it is required for histone pre-mRNA 3'-end processing in a Drosophila nuclear extract. The 71-nt Dro...
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In animals, replication-dependent histone genes are expressed in dividing somatic cells during S phase to maintain chromatin condensation. Histone mRNA 3'-end formation is an essential regulatory step producing an mRNA with a hairpin structure at the 3'-end. This requires the interaction of the U7 small nuclear ribonucleoprotein particle (snRNP) with a purine-rich spacer element and of the hair...
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Small nuclear RNAs are generally transcribed as precursors in the eukaryotic nucleus which then rapidly enter the cytoplasm. In the cytoplasm the precursors undergo RNA processing events and they also associate with specific proteins to form RNP particles or snRNPs. The RNA processing events include: (a) the addition of a tri-methyl G cap, the precursor being synthesized with the normal mono-me...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1993
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.90.13.6257